Thymosin Beta-4
Also known as: TB-4 · Tβ4 · Thymosin β4
A naturally occurring actin-binding peptide studied for wound healing, tissue repair, and cell migration.
Thymosin Beta-4 at a glance
| Name | Thymosin Beta-4 |
|---|---|
| Also known as | TB-4, Tβ4, Thymosin β4 |
| Category | Recovery & tissue repair |
| Type | Peptide |
| Sequence | Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser |
| Typical dose | ~2-5 mg per week |
| Frequency | Split across two or more doses per week |
| Route | Subcutaneous injection (research) |
| Half-life | Longer than many small peptides; systemic exposure reported over roughly a day. |
Overview
Thymosin Beta-4 is a 43-amino-acid peptide found in many tissues and body fluids, where it acts primarily as a regulator of actin, a key building block of the cell's cytoskeleton. It is commonly cited in the research literature for context on cell migration, angiogenesis, and wound repair. The peptide TB-500 is a related fragment often discussed alongside it. Most evidence comes from cell and animal studies; robust human clinical data remain limited.
How it’s thought to work
- Binds and sequesters G-actin, influencing cytoskeletal dynamics and cell migration.
- Appears to promote angiogenesis and endothelial cell movement.
- Studied for modulation of inflammation and reduction of scar formation.
- Reported to support recruitment of progenitor cells to sites of injury.
Researched uses
- Soft-tissue and muscle repair in animal models
- Corneal and dermal wound healing research
- Cardiac tissue repair studies
- Reduction of fibrosis and scarring in preclinical work
Commonly cited protocol
| Typical dose | ~2-5 mg per week |
|---|---|
| Frequency | Split across two or more doses per week |
| Route | Subcutaneous injection (research) |
| Notes | Loading and maintenance phases are sometimes described in the literature. |
Reconstitution
Commonly a 5 mg vial; 5 mg in 2 mL gives 2.5 mg/mL, so a 2 mg dose is about 32 units on a U-100 syringe.
Thymosin Beta-4 dosage calculator
The calculator below is pre-filled with Thymosin Beta-4’s commonly cited vial size and dose. Adjust the vial amount, bacteriostatic water, or dose to match your own vial — it will show the exact insulin-syringe units to draw, the concentration, and how many doses the vial holds.
Add 2 mL of bacteriostatic water to your 5 mg vial. That gives you about 2 doses of 2000 mcg. For each dose, draw 80 units into a 100-unit insulin syringe.
See the full Thymosin Beta-4 dosing chart (units for every dose & water amount), or open the full peptide calculator →
Cautions
- Not FDA-approved; sold only as a research chemical, not for human use.
- Prohibited in sport by WADA.
- Human clinical evidence is limited.
- Purity and content vary by source.
Thymosin Beta-4 FAQ
What is Thymosin Beta-4?
A naturally occurring actin-binding peptide studied for wound healing, tissue repair, and cell migration.
How does Thymosin Beta-4 work?
Binds and sequesters G-actin, influencing cytoskeletal dynamics and cell migration. Appears to promote angiogenesis and endothelial cell movement. Studied for modulation of inflammation and reduction of scar formation. Reported to support recruitment of progenitor cells to sites of injury.
What is Thymosin Beta-4 used for?
Soft-tissue and muscle repair in animal models; Corneal and dermal wound healing research; Cardiac tissue repair studies; Reduction of fibrosis and scarring in preclinical work.
What is a typical Thymosin Beta-4 dose?
~2-5 mg per week, Split across two or more doses per week, Subcutaneous injection (research). This is a commonly cited figure for context only, not medical advice.
How do you reconstitute Thymosin Beta-4?
Commonly a 5 mg vial; 5 mg in 2 mL gives 2.5 mg/mL, so a 2 mg dose is about 32 units on a U-100 syringe.
What is the half-life of Thymosin Beta-4?
Longer than many small peptides; systemic exposure reported over roughly a day.