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Recovery & tissue repair

TB-500

Also known as: Thymosin Beta-4 fragment · TB4

A synthetic version of a thymosin beta-4 region studied for systemic healing, flexibility, and recovery.

TB-500 at a glance

NameTB-500
Also known asThymosin Beta-4 fragment, TB4
CategoryRecovery & tissue repair
TypePeptide
SequenceSynthetic fragment of thymosin beta-4
Typical dose~2–2.5 mg
FrequencyA couple times weekly (loading), then weekly
RouteSubcutaneous injection
Half-lifeLonger-acting than many peptides; dosed a few times weekly.

Overview

TB-500 is based on thymosin beta-4, a naturally occurring protein involved in cell migration and tissue repair. In animal research it is associated with faster healing of muscle, tendon, and other tissues, and improved flexibility. It is popular alongside BPC-157 as a “recovery stack,” though human clinical data are limited.

How it’s thought to work

  • Regulates actin, helping cells migrate to sites of injury.
  • Promotes new blood-vessel growth (angiogenesis) and tissue remodeling.
  • Has a systemic, whole-body distribution.

Researched uses

  • Soft-tissue and muscle recovery
  • Tendon/ligament healing
  • Flexibility and injury research

Commonly cited protocol

Typical dose~2–2.5 mg
FrequencyA couple times weekly (loading), then weekly
RouteSubcutaneous injection
NotesOften cycled and stacked with BPC-157.

Reconstitution

Often a 10 mg vial. 10 mg with 2 mL water = 5 mg/mL, so a 2 mg dose = 0.4 mL (40 units on a U-100 syringe).

TB-500 dosage calculator

The calculator below is pre-filled with TB-500’s commonly cited vial size and dose. Adjust the vial amount, bacteriostatic water, or dose to match your own vial — it will show the exact insulin-syringe units to draw, the concentration, and how many doses the vial holds.

mg
2 mg = 2,000 mcg
mL
How much water you add is your choice — it doesn’t change your dose, only how many units you draw. Common:
Draw to
40 units
= 0.4 mL on a 1 mL (100 units) syringe
010203040506070809010040 ufill to the 40 u line on a 100-unit barrel
Concentration
5 mg/mL
Per unit
50 mcg/u
Doses per vial
5 × 2mg
Volume
0.4 mL
📋 In plain English

Add 2 mL of bacteriostatic water to your 10 mg vial. That gives you about 5 doses of 2 mg. For each dose, draw 40 units into a 100-unit insulin syringe.

See the full TB-500 dosing chart (units for every dose & water amount), or open the full peptide calculator →

Cautions

  • Not approved for human use; WADA-banned in sport.
  • Limited human safety data.
  • Theoretical caution with active cancer given pro-angiogenic effects.

TB-500 FAQ

What is TB-500?

A synthetic version of a thymosin beta-4 region studied for systemic healing, flexibility, and recovery.

How does TB-500 work?

Regulates actin, helping cells migrate to sites of injury. Promotes new blood-vessel growth (angiogenesis) and tissue remodeling. Has a systemic, whole-body distribution.

What is TB-500 used for?

Soft-tissue and muscle recovery; Tendon/ligament healing; Flexibility and injury research.

What is a typical TB-500 dose?

~2–2.5 mg, A couple times weekly (loading), then weekly, Subcutaneous injection. This is a commonly cited figure for context only, not medical advice.

How do you reconstitute TB-500?

Often a 10 mg vial. 10 mg with 2 mL water = 5 mg/mL, so a 2 mg dose = 0.4 mL (40 units on a U-100 syringe).

What is the half-life of TB-500?

Longer-acting than many peptides; dosed a few times weekly.

Stacks with TB-500

Related recovery & tissue repair peptides

⚠️ Educational reference only — not medical advice. These compounds are largely research chemicals not approved for human use in most countries. Nothing here is a recommendation to obtain or administer any substance. Consult a qualified clinician.