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Cognitive & neuroprotective

PE-22-28

Also known as: Spadin analog · PE 22-28

A short peptide analog of spadin, commonly cited in the research literature for context around mood and neuroplasticity.

PE-22-28 at a glance

NamePE-22-28
Also known asSpadin analog, PE 22-28
CategoryCognitive & neuroprotective
TypePeptide
SequenceSeven-residue analog of spadin, derived from the sortilin propeptide
Typical dose~200-600 mcg (figures extrapolated from animal work)
FrequencyOnce daily in studies
RouteSubcutaneous injection (research)
Half-lifeReported to be short (minutes to a few hours).

Overview

PE-22-28 is a truncated analog of spadin, a peptide fragment from the sortilin propeptide. It has been studied in animal models as a blocker of the TREK-1 potassium channel, a target linked to mood regulation. There is no established human use, and it is presented here for educational context only.

How it’s thought to work

  • Reported to inhibit the TREK-1 (two-pore potassium) channel.
  • Appears to promote hippocampal neurogenesis in models.
  • Studied for rapid antidepressant-like effects in rodents.

Researched uses

  • Depression-like behavior in animal models
  • Neurogenesis and synaptic plasticity research
  • Exploratory work on stroke and neuroprotection

Commonly cited protocol

Typical dose~200-600 mcg (figures extrapolated from animal work)
FrequencyOnce daily in studies
RouteSubcutaneous injection (research)
NotesHuman dosing is not established; some research used intranasal delivery.

Reconstitution

For a 5 mg vial reconstituted with 2 mL, the concentration is 2.5 mg/mL, so a 250 mcg dose is about 10 units on a U-100 syringe.

PE-22-28 dosage calculator

The calculator below is pre-filled with PE-22-28’s commonly cited vial size and dose. Adjust the vial amount, bacteriostatic water, or dose to match your own vial — it will show the exact insulin-syringe units to draw, the concentration, and how many doses the vial holds.

mg
mL
How much water you add is your choice — it doesn’t change your dose, only how many units you draw. Common:
Draw to
10 units
= 0.1 mL on a 1 mL (100 units) syringe
10 units0100
Concentration
2.5 mg/mL
Per unit
25 mcg/u
Doses per vial
20 × 250mcg
Volume
0.1 mL
📋 In plain English

Add 2 mL of bacteriostatic water to your 5 mg vial. That gives you about 20 doses of 250 mcg. For each dose, draw 10 units into a 100-unit insulin syringe.

See the full PE-22-28 dosing chart (units for every dose & water amount), or open the full peptide calculator →

Cautions

  • Not FDA-approved; a research chemical.
  • No human clinical evidence is available.
  • Dosing is speculative and extrapolated.
  • Purity varies by supplier.

PE-22-28 FAQ

What is PE-22-28?

A short peptide analog of spadin, commonly cited in the research literature for context around mood and neuroplasticity.

How does PE-22-28 work?

Reported to inhibit the TREK-1 (two-pore potassium) channel. Appears to promote hippocampal neurogenesis in models. Studied for rapid antidepressant-like effects in rodents.

What is PE-22-28 used for?

Depression-like behavior in animal models; Neurogenesis and synaptic plasticity research; Exploratory work on stroke and neuroprotection.

What is a typical PE-22-28 dose?

~200-600 mcg (figures extrapolated from animal work), Once daily in studies, Subcutaneous injection (research). This is a commonly cited figure for context only, not medical advice.

How do you reconstitute PE-22-28?

For a 5 mg vial reconstituted with 2 mL, the concentration is 2.5 mg/mL, so a 250 mcg dose is about 10 units on a U-100 syringe.

What is the half-life of PE-22-28?

Reported to be short (minutes to a few hours).

Related cognitive & neuroprotective peptides

⚠️ Educational reference only — not medical advice. These compounds are largely research chemicals not approved for human use in most countries. Nothing here is a recommendation to obtain or administer any substance. Consult a qualified clinician.